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Antibodies bound to the blocking peptide no more bind towards the epitope on the focus on protein. By evaluating the staining from your blocked antibody versus the antibody by yourself, one can see which staining is distinct.
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This guide outlines even further doable optimization of cellular and immunoassay parameters to be certain the best possible effects are...
This guide outlines even more probable optimization of cellular and immunoassay parameters to make certain the very best results are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind precisely for the goal antibody and block antibody binding. These peptide ordinarily includes the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress as a result of S period.
Overexpression of this gene has long been noticed in many tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital job in advertising and marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two further splice variants were claimed but specific nucleotide sequence data just isn't but obtainable.
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